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To study the composition of silkworm pupae oil, lipid of silkworm pupae was separated into two fractions, ether extractable and 85% methanol extractable, and the lipid components of each fraction were analyzed by using silicic acid column, thin layer chromatography and gas chromatography. Silkworm pupae contains 35.4% crude fat (dry-basis) of which consists 34.4% diethyl ether-extract and 0.9% of 85% methanol-extract. The diethyl ether-extract contained 96.1% of neutral lipid, 2.9% of glycolipid and 1.0% of phospholipid while methanol-extract was consisted of 47.4% of neutral lipid, 14.6% of glycolipid and 38.1% of phospholipid. The major components of phospholipid were phosphatidyl glycol(41.0%), and phosphatidyl choline(28.2%) and phosphatidyl ethanolamine(21.2%) in the diethyl ether-extract and phosphatidyl glycol (48.4%), phosphatidyl inositol(22.8%) arid phosphatidyl choline(17.9%) in the methanol-extract. The major fatty acids of the total lipid were oleic acid(33.5%), linolcnic acid(31.0%) and palmitic acid(23.1%).
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